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Master thesis biology - microbiome research (m/f/d)

Braunschweig
Abschlussarbeit
Helmholtz-Zentrum für Infektionsforschung
Master
405 € - 495 € pro Monat
Inserat online seit: 25 Juli
Beschreibung

As a member of the Helmholtz Association, the Helmholtz Centre for Infection Research, based in Braunschweig, conducts top-level research in the field of infectious diseases. Our scientists develop novel methods and strategies to fight infectious diseases faster and more effectively. Our common goal is to develop novel approaches for the prevention, diagnosis and treatment of infectious diseases. Our research focuses on bacterial and viral pathogens, their interaction with the human immune system and new effective substances and vaccines.

The department Microbial Immune Regulation of Prof. Dr. Till Strowig at the Helmholtz Centre for Infection Research (HZI) in Braunschweig, Germany is offering a Master Thesis in the area of human microbiome research:

“Characterization of Phage-Bacteria Dynamics in the Human Gut Ecosystem”


The human gut microbiome is a complex and dynamic ecosystem comprising trillions of microorganisms, including bacteria, viruses and fungi. It plays a vital role in maintaining health by contributing to digestion, regulating the immune system, and protecting against pathogens. Disruptions to this microbial balance, known as dysbiosis, are associated with various diseases, including infections, inflammatory conditions and metabolic disorders.

One group of opportunistic pathogens that can thrive during gut dysbiosis are various Klebsiella species. These bacteria can colonize the gut without causing symptoms, but they can also cause severe infections, particularly in individuals with weakened immune systems or following antibiotic treatment. Some Klebsiella strains produce cytotoxins or carry antibiotic resistance genes, making them a growing concern in hospital and community settings alike. Bacteriophage therapy, which employs viruses that specifically infect bacteria, is a promising alternative to antibiotics for treating Klebsiella infections. Phages can target pathogenic strains with high specificity while sparing the beneficial microbiota. In the context of the gut, phage therapy may help to restore microbial balance by selectively eliminating harmful bacteria. This offers a precise way of managing infections and reducing reliance on broad-spectrum antibiotics. The objective of this study is to investigate the interactions and dynamics between bacteriophages and bacteria in the human gut ecosystem. To this end, an integrated in vitro and in vivo approach will be employed to enhance our understanding of their ecological roles and potential health implications.

Methods:

Phage quantification via spotting and plating assays
Methods for determining the host range and specificity of phages

Ex vivo assays with different microbiota samples, including from mice
Culturing of intestinal bacteria
Various in vitro competition assays between phages and bacteria
Assistance with in vivo experiments (mice and Galleria mellonella)




Advanced masters programm
An expense allowance of EUR 450 per month for a maximum of 6 months
An exciting and varied role in a future-oriented research institute with an international environment on the Science Campus South in Braunschweig
Access to state-of-the-art infrastructure and the latest technologies
A corporate culture that promotes appreciation and equal opportunities
Professional supervision and technical support during your master's thesis
The opportunity to work on a current and practice-relevant topic in a scientific context and learn a lot in a friendly and international team

If equally qualified, severely disabled persons will be given preference. To protect your rights, please clearly indicate any severe disability in your cover letter or CV.
The HZI is committed to professional equality between women and men, and we therefore expressly welcome applications from qualified women.

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