PhD Student (gn*) Physiological Chemistry and Pathobiochemistry 19.12.2025 Universitätsklinikum Münster Münster
Weitere passende Anzeigen:
Jobmailer
Ihre Merkliste /
Mit Klick auf einen Stern in der Trefferliste können Sie sich die Anzeige merken
1
Passende Jobs zu Ihrer Suche ...
... immer aktuell und kostenlos per E-Mail.
Sie können den Suchauftrag jederzeit abbestellen.
Es gilt unsere Datenschutzerklärung. Sie erhalten passende Angebote per E-Mail. Sie können sich jederzeit wieder kostenlos abmelden.
PhD Student (gn*) Physiological Chemistry and Pathobiochemistry
Drucken
Teilen
Münster
Informationen zur Anzeige:
PhD Student (gn*) Physiological Chemistry and Pathobiochemistry
Münster
Aktualität: 19.12.2025
Anzeigeninhalt:
19.12.2025, Universitätsklinikum Münster
Münster
PhD Student (gn*) Physiological Chemistry and Pathobiochemistry
Über uns:
We are UKM. We have a clear social mission and, with our focus on healthcare, research, and teaching, we bear a unique responsibility. Marie Sklodowska-Curie Action Doctoral Network (MSCA-DN) REMOD-HEALING: «Targeting Extracellular Matrix Remodelling in Cutaneous Wounds to Promote Better Healing» REMOD-HEALING is a research network of leading European and Latin American scientists from academia and industry who want to tackle the burden of pathological wounds by exploiting therapeutic targets and biomarkers associated with extracellular matrix remodelling in cutaneous wounds. Through its research and training activities, the REMOD-HEALING project will contribute to scientific advancement and innovation in wound healing and regenerative medicine through a coordinated, interdisciplinary effort, ultimately leading to societal and economic benefits. The following project is offered at the University of Münster: Mapping and quantification of collagenase activity in protease-damaged tissue; Subject area: Vascular and stromal ECM, cell adhesion, recombinant production of ECM and mammalian collagenases, protein chemical modifications, protease assays, (in-situ) zymography, CD spectroscopy, FRET and FLIM The University Hospital of Münster is one of the leading hospitals in Germany. Such a position cannot be achieved by size and medical successes alone. The individual commitment counts above all. We need your commitment so that even with little things we can achieve great things for our patients. There are many possibilities open for you so that you may develop with them.
Aufgaben:
* Generate collagenase-sensitive probes whose cleavage site depends on native triple-helical conformation of collagen
* Equip these collagenase-sensitive probes with FRET (Förster resonance energy transfer)- and FLIM (fluorescence life time microscopy)-capable molecular moieties, which enable fluorescence-based real-time monitoring of their cleavage
* Determine characteristic features of the probes, such as thermal stability of the triple-helical conformation as well as affinity and selectivity for different proteases (e.g. collagenolytic and gelatinolytic matrix metalloproteinases (MMPs) and snake venom metalloproteinases (SVMPs)
* Use the probes to localize and quantify collagenase activity in damaged tissue (tissue samples of snake bites and other skin wounds by zymography)
* Use the probes to assess the inhibition of collagenase-targeting tools on different collagenases
* Develop non-cleavable homologs of the collagenase-sensitive probes that block the active site of collagenases
* EXPECTED RESULTS:
* Develop novel, diagnostic tools for mapping and quantifying collagenase activity within wounded tissue
* A test to determine collagenase activity as a marker of (aberrant) wound healing
* This study will also bring forth lead structures based on triple-helical collagen structures that may serve as collagenase inhibitor that can also be used in mechanistic studies
* Acquisition of common methods of molecular biology (e.g. cloning, PCR), of protein expression, purification and modification, of protein-chemical analysis and detection, of biophysical measurement (e.g. circular dichroism, fluorescence polarisation, FRET analysis) and of microscopy (e.g. fluorescence microscopy, laser scanning microscopy, video microscopy), as well as of cell culture technology (e.g. adhesion, migration, and invasion assays)
* Part of Research Innovation
Qualifikationen:
* Master's degree in biology, biochemistry or chemistry
* Experience in protein biochemistry, molecular biology and/or cell biology
* Willingness to learn new methods and technologies in an international and intercultural team environment
Wir bieten:
* A 36-month project as Marie Sklodowska-Curie trainee in one of the participating institutions with the objective of receiving a doctoral degree (PhD)
* State-of-the-art research in an international consortium with highly integrated research projects
* Expert training in basic and applied research, along with a thorough understanding of the process involved in transitioning from basic to pre-clinical research
* Research training periods in another consortium member's lab
* Training in both academic and industrial research environments
* Salary according to EU guidelines for Marie Sklodowska-Curie trainees, including mobility payments and family allowances where applicable
* 3-month secondment at CNRS Lyon, France, in the lab of Dr. Catherine Moali for fluorescence polarisation measurements of collagenolytic activity
* 3-week placement at Nordic Bioscience, Herlev, Denmark, in the lab of Dr. Signe Holm Nielsen for testing the collagenase activity probes as an applicable biomarker assay
* Professional Development and Training
* Exciting Projects
Weitere Informationen:
Questions about how to apply? Click here Equal opportunities are a part of our Human Resources Policy. UKM supports the compatibility of work and family life and is certified as "Family-friendly Company" since 2010. Applications of women are specifically invited. In the case of similar qualifications, competence, and specific achievements, women will be considered on preferential terms within the framework of the legal possibilities. Handicapped candidates with equivalent qualifications will be given preference. Due to legal requirements working with us is only permitted with complete vaccination against measles.
Berufsfeld
Bundesland
Standorte
©