Your university hospital in the heart of Munich We are the TUM Klinikum Rechts der Isar and a reliable employer for around 6,600 employees. As a university hospital, we are dedicated not only to patient care but also to research and teaching - in line with our mission statement "Knowledge creates healing" and enjoy an excellent reputation both nationally and internationally. 160 different professional groups work hand in hand to provide our patients with optimal treatment. PhD Student - Life Science, Biology, Immunology As part of the Research Training Group RTG 2668 -Immune Master Switches in Allergies and Autoimmune Diseases-, the research group -Airway Immunology- led by Prof. Dr. Carsten Schmidt-Weber and PD Dr. Constanze Jakwerth is looking for a PhD Student. The translational project investigates key factors of the innate immune system that control the development of tolerance to allergen-specific immunotherapy (AIT) at the immune system level and at the muscosal airway barrier. State-of-the-art technology (including high-dimensional flow cytometry, transcriptome analyses, proteomics) will be used to identify and functionally characterise human T cell subtypes, molecular signalling pathways and new adjuvant agents You will establish human 3D and tissue cultures Your tasks include immunofluorescence staining, cofocal microscopy and image analysis Your work includes molecular biological and epigenetic analyses You will carry out individual analyses such as high-dimensional flow cytometry or single cell sequencing You will analyse immune mediators at RNA and protein level You will independently plan, carry out and analyse experiments You already have a degree in the field of life sciences, biology, biochemistry, molecular medicine or molecular biotechnology You have already gained practical experience with molecular biological, immunological or cell biological methods You are enthusiastic about translational immunology and tolerance mechanisms in allergic and inflammatory diseases EGYM well pass, corporate benefits and discounts (e.g. Käfer), cafeteria, sports and cultural offers, company bike Free use of the library through a branch of the Munich City Library located in the building Working in the heart of Munich at Max-Weber-Platz with very good accessibility by public transport such as the subway, S-Bahn or tram Company pension plan through the Federal and State Pension Institute (VBL) Self-realization through a varied and technically demanding job with interdisciplinary cooperation between various departments of the TUM Klinikum