Recurrent copy-number alterations (CNAs) are a well-described feature of pediatric brain tumors such as high-grade glioma and medulloblastoma. Our recent findings indicate that subtype-specific CNAs play an essential role in tumorigenesis for many entities. Additionally, we have now found that somatic mouse models established in our groups closely mimic patient tumors and harbor recurrent CNAs that are syntenic to their human counterparts. The PhD student will use our robust mouse model platform to investigate the detailed molecular role of CNAs during solid tumor initiation, progression, and evolution.
Your Tasks
The PhD student will:
* Establish when CNAs arise
* Identify gene expression
* Analyze chromatin accessibility changes in individual tumor cells over time using single-cell RNA and ATAC sequencing
* Pinpoint the occurrence of CNAs on a genomic level
* Analyze clonality and gene expression changes to identify putative functional consequences of early CNAs using single-cell whole-genome (ResolveOME) sequencing
Therefore, the project initially focuses on bioinformatic analyses but offers opportunity to work in the wet lab and with animal models at later stages. In addition to his/her own project, the PhD student would work in diverse collaborations with other students and postdocs.
Your Profile
* Completed master's or diploma degree in biology, bioinformatics, or a related field
* Background knowledge of tumor biology, particularly brain tumors, as well as experience in bioinformatics is beneficial (knowledge of Linux/Unix command line and R, experience in 1-2 programming languages, familiarity with job submission on computer clusters)
* Highly motivated and passionate about developing meaningful projects independently within a supportive team environment
* Excellent communication skills
* Reliability, attention to detail
* Thoughtfulness regarding experiments and colleagues is expected
* Experience with molecular biology laboratory methods and a willingness to work with animal models is beneficial but is not essential
* Fluency in verbal and written English
We Offer
Excellent framework conditions: state-of-the-art equipment and opportunities for international networking at the highest level
Access to international research networks
Doctoral salary with the usual social benefits
30 days of vacation per year
Flexible working hours
Possibility of mobile work and part-time work
Family-friendly working environment
Sustainable travel to work: subsidized Germany job ticket
Unleash your full potential: targeted training and mentoring through the DKFZ International PhD Program and DKFZ Career Service
Our Corporate Health Management Program offers a holistic approach to your well-being