About the Job Are you ready to shape the future of medicine? The race is on to speed up drug discovery and development to find answers for patients and their families. Your skills could be critical in helping our teams accelerate progress. The department of Translational In Vivo Models (TIM) within Sanofi's R&D in Frankfurt, Germany, is seeking a highly motivated Principal Scientist with a strong background in immunology, molecular biology, and mouse genetics. In this role, you will carry out molecular and immunological characterization of genetically engineered rodent models that are crucial for early phases of drug development. Join our team and play a pivotal role in validating transgenic models that drive breakthrough discoveries in medicine. About Sanofi: We're an R&D-driven, AI-powered biopharma company committed to improving people's lives and delivering compelling growth. Our deep understanding of the immune system - and innovative pipeline - enables us to invent medicines and vaccines that treat and protect millions of people around the world. Together, we chase the miracles of science to improve people's lives. Main Responsibilities Model Characterization and Validation Conduct molecular and immuno-phenotypic characterization studies of genetically modified rodent models using state-of-the-art equipment and methodologies Design, organize, and execute validation experiments for new transgenic models, ensuring alignment with project milestones and timelines Collaborate closely with principal investigators, project pharmacologists, and Sanofi researchers to define and meet specific research project needs Technical Expertise and Analysis Perform advanced multicolor spectral flow cytometry (15 colors) including panel design, optimization, and critical data interpretation Conduct unsupervised flow cytometry analysis using platforms such as OMIQ and FlowJo Characterize diverse immune and non-immune cell sub-populations by cytometry Culture murine primary cells including isolation (magnetic and FACS cell sorting), culture, activation, and differentiation Data Management and Reporting Prepare comprehensive reports and presentations for internal and external stakeholders Ensure quality and integrity of all data collected through proper compilation and entry into database systems Manage electronic laboratory notebooks and maintain rigorous documentation standards Apply statistical analysis methods using Prism and other relevant software Strategic Collaboration Maintain interactive working relationships with model providers, CROs, colleagues, and key stakeholders Provide expert guidance on transgenic research models and recommend optimal validation strategies Share knowledge through monthly oral presentations and collaborative discussions with multidisciplinary teams Implement and recommend new procedures to enhance model development processes Laboratory Operations Conduct mice experimentation at Designer level: genotyping, breeding, injections (IP, IV), in vivo immunization, and sample processing Follow HSE requirements to implement and expand laboratory activities Contribute to laboratory management and best practice development About You Required Qualifications Education: PhD in immunology, molecular biology, mouse genetics, or related biological sciences Experience: Minimum 5 years of hands-on experience in multicolor spectral flow cytometry (minimum 15 colors), including panel design, optimization, analysis, and critical interpretation Extensive experience with spectral cytometers (Cytek, BD, Sony, or similar platforms) Proven expertise in unsupervised flow cytometry analysis (OMIQ, FlowJo, or equivalent) - mandatory Demonstrated proficiency in statistical analysis using Prism - mandatory Strong background in mouse molecular genetics and immuno-phenotyping Experience in project management: designing, organizing, and executing experiments aligned with project objectives Technical Skills: Expert-level knowledge in immunology and biological sciences Proficiency in culturing murine primary cells and performing cell isolation techniques Competency in mice experimentation (Designer level) including genotyping, breeding, and various injection routes Strong computer skills including Microsoft Office Suite (Word, PowerPoint, Excel) and database management systems Languages: English: Full professional proficiency required Desired Attributes Professional Competencies: Excellent organizational and communication skills Ability to lead and work collaboratively within multidisciplinary teams Strong motivation to contribute to developing genetically modified preclinical animal models Proactive approach to implementing innovative procedures and methodologies Demonstrated ability to manage multiple projects and meet deadlines Personal Qualities: Detail-oriented with commitment to data quality and integrity Self-motivated with ability to work independently and as part of a team Strong problem-solving skills and scientific curiosity Commitment to continuous learning and professional development Why Choose Us? Bring the miracles of science to life alongside a supportive, future-focused team dedicated to transforming patient outcomes Discover endless opportunities to grow your talent and drive your career, whether through promotion or lateral moves, at home or internationally Enjoy a thoughtful, well-crafted rewards package that recognizes your contribution and amplifies your impact Take good care of yourself and your family with a wide range of health and wellbeing benefits including high-quality healthcare, prevention and wellness programs, and at least 14 weeks' gender-neutral parental leave Work with cutting-edge technology in an excellent laboratory environment with motivated staff and state-of-the-art equipment Contribute to groundbreaking research in transgenic models that directly impact drug discovery and development Join a world-class R&D hub in Frankfurt, a vibrant international city with excellent quality of life and strong scientific community LI-Onsite Immunology TransgenicModels Sanofi SanofiCareers PursueProgress DiscoverExtraordinary IgnitingPotential Diversity und Inklusion sind in den Grundwerten von Sanofi verankert und spiegeln sich in unserer Arbeitsweise wider. Wir respektieren die Vielfalt unserer Belegschaft in Hinsicht auf ihre Herkunft, Erfahrungen und Lebensweisen. Wir erkennen die Bereicherung, die diese Vielfalt birgt, und fördern Inklusion sowie eine Arbeitsumgebung, in der diese Unterschiede sich weiter entwickeln können, zur Stärkung des Lebens unserer Mitarbeiter, Patienten und Kunden.